EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.122 | overexpression in Escherichia coli | Leptosphaeria maculans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.122 | EDTA | 5 mM, 12% inhibition | Leptosphaeria maculans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.122 | 0.13 | - |
L-tyrosine | pH 7.5, 37°C | Leptosphaeria maculans | |
2.5.1.122 | 0.17 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Leptosphaeria maculans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.122 | Ca2+ | 5 mM, 1.4fold activation compared to activity without additives | Leptosphaeria maculans | |
2.5.1.122 | additional information | no requirement for divalent metal ion | Leptosphaeria maculans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.122 | 50000 | - |
2 * 50000, SDS-PAGE | Leptosphaeria maculans |
2.5.1.122 | 100000 | - |
His6-tagged enzyme, gel filtration | Leptosphaeria maculans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | Leptosphaeria maculans | the enzyme is involved in biosynthesis of the phytotoxin sirodesmin PL | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.122 | Leptosphaeria maculans | Q6Q874 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.122 | - |
Leptosphaeria maculans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.122 | dimethylallyl diphosphate + L-3,4-dihydroxyphenylalanine | 16.6% of the activity, compared to L-tyrosine. It is expected that the prenylation takes place at the 4-hydroxyl group. The structure of the product is not determined | Leptosphaeria maculans | ? | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | the enzyme is involved in biosynthesis of the phytotoxin sirodesmin PL | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | no activity with cyclo-L-Tyr-L-Ser, L-Phe, 4-hydroxybenzoate or 4-coumarate | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
2.5.1.122 | additional information | SirD also prenylates L-Trp, resulting in the formation of 7-dimethylallyltryptophan. SirD catalyses the C-prenylation of L-Trp, in addition to the O-prenylation of L-Tyr, cf. EC 2.5.1.34 | Leptosphaeria maculans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.122 | dimer | 2 * 50000, SDS-PAGE | Leptosphaeria maculans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.122 | SirD | - |
Leptosphaeria maculans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.122 | 37 | - |
assay at | Leptosphaeria maculans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.122 | 1 | - |
L-tyrosine | pH 7.5, 37°C | Leptosphaeria maculans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.122 | 7.5 | - |
assay at | Leptosphaeria maculans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.122 | physiological function | the enzyme is involved in biosynthesis of the epidithiopiperazinedione toxin sirodesmin PL by the phytopathogenic ascomycete Leptosphaeria maculans | Leptosphaeria maculans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.122 | 7.7 | - |
L-tyrosine | pH 7.5, 37°C | Leptosphaeria maculans |